Work

Robust Light Emission from Cyclic Alkylaminoluciferin Substrates for Firefly Luciferase

Year: 2010

Type: article

Abstract: Firefly luciferase utilizes the chemical energy of ATP and oxygen to convert its substrate, D-luciferin, into an excited-state oxyluciferin molecule. Relaxation of this molecule to the ground state is responsible for the yellow-green light emission. Synthetic cyclic alkylaminoluciferins that allow robust red-shifted light emission with the modified luciferase Ultra-Glo are described. Overall light emission is higher than that of acyclic alkylaminoluciferins, aminoluciferin, and the native substrate D-luciferin.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja104525m

Authors: Gadarla Randheer Reddy, Walter C. Thompson, Stephen C. Miller

Institution: University of Massachusetts Chan Medical School

Language: English

Topic: bioluminescence and chemiluminescence research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Cancer Institute

Award: Near-IR Bioluminescence Imaging