Work

Distribution of Lymphocyte Messenger RNA during Stimulation by Phytohaemagglutinin

Year: 1979

Type: article

Abstract: Additional of phytohaemagglutinin to cultured lymphocytes results in a progressive increase in the rate of protein synthesis of up to 7-10 times the initial rate after 20 h. Between 2 h and 12 h after the addition of mitogen, the increase in the rate of protein synthesis can be accounted for by the transfer of mRNA from messenger ribonucleoprotein complexes to polysomes. Between 12 h and 20 h the increase also reflects the accumulation of mRNA and ribosomes. However, the proportion of mRNA associated with ribosomes in unstimulated lymphocytes is unexpectedly high and paradoxically decreases during the first 2 h after mitogen addition, although the rate of protein synthesis increases. A mechanism involving mRNA selection is suggested.

Source: European Journal of Biochemistry

DOI: 10.1111/j.1432-1033.1979.tb04195.x

Authors: Rosemary JAGUS, John E. KAY

Institutions: National Institutes of Health, National Heart, Lung, and Blood Institute, University of Sussex

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funder: Medical Research Council

OpenAlex ID: W1979090379

FWCI: 1.066

Cites: 30

Cited by: 22

Related to: 10