Work

Multilayer DNA Origami Packed on Hexagonal and Hybrid Lattices

Year: 2011

Type: article

Abstract: "Scaffolded DNA origami" has been proven to be a powerful and efficient approach to construct two-dimensional or three-dimensional objects with great complexity. Multilayer DNA origami has been demonstrated with helices packing along either honeycomb-lattice geometry or square-lattice geometry. Here we report successful folding of multilayer DNA origami with helices arranged on a close-packed hexagonal lattice. This arrangement yields a higher density of helical packing and therefore higher resolution of spatial addressing than has been shown previously. We also demonstrate hybrid multilayer DNA origami with honeycomb-lattice, square-lattice, and hexagonal-lattice packing of helices all in one design. The availability of hexagonal close-packing of helices extends our ability to build complex structures using DNA nanotechnology.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja209719k

Authors: Yonggang Ke, Niels V. Voigt, Kurt V. Gothelf, William M. Shih

Institutions: Harvard University, Dana-Farber Cancer Institute, Aarhus University, Danish National Research Foundation, Wyss Institute for Biologically Inspired Engineering

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

Award: NMR Structure Determination of Membrane Proteins Enabled by DNA Nanotubes