Work

Application of gene probes to detect a penaeid shrimp baculovirus in fixed tissue using in situ hybridization

Year: 1993

Type: article

Abstract: DNA inserts obtained from a cloning of the penae~d shrimp baculovirus, BP, were nonradioactively labelled with digoxigenin for use as gene probes to detect BP in paraffin-embedded, formalin/alcohol-fixed tissue sections using in sit11 hybridization.Results show that the probes detect BP in various species of shrimp from different geoglaphicdl dreas.The probes demonstrate no reaction to BP-negative tissue sections.The probes also demonstrate no reaction to tissue infected with other penaeid shrimp viruses.

Source: Diseases of Aquatic Organisms

DOI: 10.3354/dao017215

Authors: LD Bruce, RM Redman, DV Lightner, JR Bonami

Institutions: University of Arizona, Université de Montpellier

Language: English

Topic: Aquaculture disease management and microbiota

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W1979582118

FWCI: 1.544

Cites: 4

Cited by: 34

Related to: 10