Work

Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction

Year: 1990

Type: article

Abstract: A hammerhead domain of less than 50 nucleotides is responsible for a self-cleavage reaction in the replication of plant RNA pathogens. The hammerhead is composed of three helices joining at a central conserved core of 11 single stranded nucleotides. The core is believed to fold into a tertiary structure that provides functional groups for catalysis and to coordinate one or more divalent metal ions. In this study we use a phosphorothioate substitution interference assay to identify four phosphates in the conserved core which also play a role in the self-cleavage reaction.

Source: Nucleic Acids Research

DOI: 10.1093/nar/18.20.6025

Authors: Duane E. Ruffner, Olke C. Uhlenbeck

Institutions: University of Colorado Boulder, University of Colorado System

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: W. M. Keck Foundation, National Institutes of Health, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases

Awards: G3859735013, DESIGN AND SELECTION OF RIBOZYMES FOR HIV THERAPY