Year: 2007
Type: article
Abstract: Telomeres terminate in 3' overhangs that function in end protection and the formation of t-loops. Determining the steps and factors involved in overhang processing is compromised by the inability to easily and accurately determine overhang size in the presence of many kilobases of double-stranded telomeric DNA. We here describe the use of a double-strand specific nuclease (DSN) that entirely digests double-stranded DNA including telomeres, leaving the overhangs intact so that they can be measured.
Source: Nucleic Acids Research
DOI: 10.1093/nar/gkm1063
Authors: Y. Zhao, H. Hoshiyama, J. W. Shay, W. E. Wright
Institution: The University of Texas Southwestern Medical Center
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,992
Funder: National Institute on Aging
Awards: G2682010215, G3182088148
OpenAlex ID: W1980263741