Work

Tight Junction–Associated Signaling Pathways Modulate Cell Proliferation in Uveal Melanoma

Year: 2010

Type: article

Abstract: PURPOSE: To investigate the role of tight junction (TJ)-associated signaling pathways in the proliferation of uveal melanoma. METHODS: Human uveal melanoma cell lines overexpressing the TJ molecule blood vessel epicardial substance (Bves) were generated. The effects of Bves overexpression on TJ protein expression, cell proliferation, and cell cycle distribution were quantified. In addition, localization and transcription activity of the TJ-associated protein ZO-1-associated nucleic acid binding protein (ZONAB) were evaluated using immunofluorescence and bioluminescence reporter assays to study the involvement of Bves signaling in cell proliferation-associated pathways. RESULTS: Bves overexpression in uveal melanoma cell lines resulted in increased expression of the TJ proteins occludin and ZO-1, reduced cell proliferation, and increased sequestration of ZONAB at TJs and reduced ZONAB transcriptional activity. CONCLUSIONS: TJ proteins are present in uveal melanoma, and TJ-associated signaling pathways modulate cell signaling pathways relevant to proliferation in uveal melanoma.

Source: Investigative Ophthalmology & Visual Science

DOI: 10.1167/iovs.10-5746

Authors: Ashwath Jayagopal, Jin-Long Yang, Frederick R. Haselton, Min S. Chang

Institutions: Vanderbilt University, Vanderbilt University Medical Center

Language: English

Topic: Ocular Oncology and Treatments

Subfield: Ophthalmology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $1,850