Work

Fluorescence of Organic Binders in Painting Cross-sections

Year: 2013

Type: article

Abstract: This research is focused on the application of immuno-fluorescence microscopy (IFM) to localize and identify proteinaceous materials in painting cross-sections. To find the best analytical protocol and the best fluorochrome, a comparison between two different fluorescent markers for the secondary antibody has been performed. An already known fluorochrome has been compared with a marker not commonly used to characterize painting materials. Parallel to IFM, staining tests by means of fluorescent selective reagents, have been performed. The study was carried out on standard and historical samples. Drawbacks and limits of the methods are here presented and solutions to overcome the encountered problems are discussed.

Source: Procedia Chemistry

DOI: 10.1016/j.proche.2013.03.025

Authors: Donata Magrini, Susanna Bracci, Irina Crina Anca Sandu

Institutions: National Research Council, Universidade Nova de Lisboa

Language: English

Topic: Cultural Heritage Materials Analysis

Subfield: Archeology

Field: Arts and Humanities

Domain: Social Sciences

Open Access status: diamond

Funders: European Commission, Ministério da Ciência, Tecnologia e Ensino Superior, Fondazione Monte dei Paschi di Siena, Fundação para a Ciência e a Tecnologia

Awards: Projecto Estratégico - LA 6 - 2011-2012, G1769701895, G2067375997, G4862636923, Cultural heritage Advanced Research Infrastructures: Synergy for a Multidisciplinary Approach to Conservation/Restoration