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Dissociation of Mammalian Polyribosomes into Subunits by Puromycin

Year: 1971

Type: article

Abstract: Hepatic ribosomes have been dissociated into biologically active subunits as follows. Polysomes were treated at 0 degrees C with puromycin at high ionic strength. This released most of the nascent polypeptide chains without dissociating the polysomes, which retained the mRNA and the tRNA moiety of peptidyl tRNA, but were unable to continue the translation of mRNA. The polysomes were then heated to 37 degrees C, when they dissociated completely into subunits. Similar treatment without puromycin resulted in only partial dissociation.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.68.2.390

Authors: Günter Blobel, David Sabatini

Institution: Rockefeller University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: U.S. Public Health Service

OpenAlex ID: W1980600234

FWCI: 11.13

Cites: 14

Cited by: 439

Related to: 10