Work

Isolation of a heparan sulfate-containing proteoglycan from basement membrane.

Year: 1980

Type: article

Abstract: We have isolated a unique, basement membrane proteoglycan from the Engelbreth-Holm-Swarm (EHS) sarcoma. This proteoglycan, estimated to be 0.75 X 10(6) daltons, was found to contain about equal amounts of protein and covalently linked heparan sulfate. Antibody prepared against this proteoglycan reacts with the basement membrane matrix in the tumor and with the basement membranes in skin, kidney, and cornea. These studies indicate that the heparan sulfate proteoglycan is a normal constituent of basement membranes that presumably plays an important role in the organization of basement membrane components and that also may determine the permeability of basement membranes to acidic molecules.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.77.8.4494

Authors: J R Hassell, P G Robey, H J Barrach, J Wilczek, S I Rennard, G R Martin

Institutions: National Institutes of Health, National Institute of Dental and Craniofacial Research, National Eye Institute

Language: English

Topic: Proteoglycans and glycosaminoglycans research

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W1980617596

FWCI: 11.14

Cites: 34

Cited by: 680

Related to: 10