Work

Capillary electrophoresis applied to proteomic analysis

Year: 2009

Type: article

Abstract: In the postgenomic era, proteomics has become a dominant field for identifying and quantifying the complex protein machinery of the cell. The expression levels, posttranslational modifications, and specific interactions of proteins control the biology of such processes as development, differentiation, and signal transduction. Studies of the proteins involved in these processes often lead to a better understanding of biology and of human disease. Powerful separation techniques and sensitive detection methods enable researchers to untangle these complicated networks of processes. CE coupled with either MS or LIF are two of the techniques that make this possible. This review will cover proven CE-based methods for proteomics on the cell and tissue level and their application in biological and clinical studies, relevant new developments in enabling technology such as microfluidic CE-MS demonstrated on model systems, and comment on the future of CE in proteomics.

Source: Journal of Separation Science

DOI: 10.1002/jssc.200800592

Authors: Bryan R. Fonslow, John R. Yates III

Institution: Scripps Research Institute

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases, National Center for Research Resources

Awards: Proteomic Analysis of C. Elegans Insulin Signaling, Comprehensive Biology: Exploiting the Yeast Genome