Work

Quantitative imaging of complex samples by spiral phase contrast microscopy

Year: 2006

Type: article

Abstract: Recently a spatial spiral phase filter in a Fourier plane of a microscopic imaging setup has been demonstrated to produce edge enhancement and relief-like shadow formation of amplitude and phase samples. Here we demonstrate that a sequence of at least 3 spatially filtered images, which are recorded with different rotational orientations of the spiral phase plate, can be used to obtain a quantitative reconstruction of both, amplitude and phase information of a complex microscopic sample, i.e. an object consisting of mixed absorptive and refractive components. The method is demonstrated using a calibrated phase sample, and an epithelial cheek cell.

Source: Optics Express

DOI: 10.1364/oe.14.003792

Authors: Stefan Bernet, Alexander Jesacher, Severin Fürhapter, Christian Maurer, Monika Ritsch-Marte

Institution: Innsbruck Medical University

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,270

Funder: Austrian Science Fund