Work

Affinity-purified interleukin 2 induces proliferation of large but not small B cells.

Year: 1985

Type: article

Abstract: Immunoaffinity-purified interleukin 2 (IL2) stimulated proliferation of large but not small B cells. Stimulation was observed even when B cells were cultured at very low cell densities (3 X 10(4) per microwell containing 0.2 ml of medium). Addition of small numbers of purified splenic T cells did not enhance the IL2-induced B-cell proliferative response. These results suggest that IL2 was not operating through contaminating T cells. B cells cultured with anti-Ig antibody in vitro showed enhanced proliferation when cultured with EL4 thymoma-derived B-cell growth factor but not when cultured with IL2. A direct role for IL2 in B-cell activation is discussed.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.82.5.1518

Authors: J J Mond, C Thompson, F D Finkelman, J Farrar, M Schaefer, R J Robb

Institutions: Naval Medical Research Command, Uniformed Services University of the Health Sciences, DuPont (United States)

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Science Foundation, Uniformed Services University of the Health Sciences, Office of Naval Research