Work

Synthetic phosphopeptide immunogens yield activation-specific antibodies to the c-erbB-2 receptor.

Year: 1992

Type: article

Abstract: We inoculated rabbits with synthetic phosphopeptides, duplicating a major autophosphorylation site of the c-erbB-2 protooncogene product. The rabbits produced antisera that, after reverse immunoaffinity purification, selectively recognize the erbB-2 protein in its enzymatically active configuration. These anti-phosphopeptide antisera identify a subset of erbB-2-positive human cell lines wherein the protein is constitutively active as a tyrosine kinase. Synthetic phosphopeptides incorporating informative protein phosphorylation sites may prove useful for generating antibodies that indicate the activation state of additional tyrosine kinases and perhaps other proteins phosphorylated on serine and threonine residues.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.89.21.10435

Authors: R J Epstein, B J Druker, T M Roberts, C D Stiles

Institutions: Dana-Farber Cancer Institute, Harvard University

Language: English

Topic: HER2/EGFR in Cancer Research

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute, Eunice Kennedy Shriver National Institute of Child Health and Human Development