Effect of DC-CIK cell on the proliferation, apoptosis and differentiation of leukemia cells
Year: 2014
Type: article
Abstract: OBJECTIVE: To observe the effect of co-culture cytokine-induced killer cells (CIK) and homologous dendritic cells (DC) on the proliferative activity and phenotype change of the DC-CIK cell and the cell killing activity of leukemia HL-60. METHODS: 50 mL cord blood sample was obtained from infants delivered by full term healthy woman and the cord blood mononuclear cells were isolated by density gradient centrifugation. Non-adherent cells were collectedfor the induction culture of CIK, adherent cells were differentiated into mature DC; cultured mature DC was mixed with and CIK in the proportion of 1:5 for 12 d. Killing activity of DC-CIK co-cultured cell on leukemia HL-60 was detected by MTT assay. RESULTS: Compared with CIKs, the co-cultured DC-CIKs presented a markedly higher proliferation and killing activity. CONCLUSIONS: Co-culture of DC-CIK cells led to a significant increase of the proliferation and cytotoxicity of CIK.
Authors: Hui-Qing Qu, Xiao-Sheng Zhou, Xiao-Long Zhou, Jian Wang
Institutions: Binzhou Medical University, Binzhou Medical College Hospital
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W1981576463