Year: 2009
Type: article
Abstract: Momordica charantia L. (Cucurbitaceae) is widely used as a traditional medicine, having antidiabetic, antitumor, antiviral activities and so on. Many triterpenoids and other components had been found from M. Charantia. In our present work, the fruit of Momordica Charantia L. were extracted by alcohol then purified by D-101 macro porous absorptive resin followed by chloroform extraction. Isolation and purification were carried out by silica gel chromatography resulting in nine compounds: three novel cucurbitane-type triterpenoids, named charantagenins A( 1 ), B( 2 ) and C( 3 ), (+)-eduesmin( 4 ) and bluemenol A( 5 ) are being reported for the first time from Momordica Charantia L., and four known compounds: karavilagenin D( 6 ), 3β,7β,25-trihydroxy-cucurbita-5, (23E)-diene-19-al( 7 ), 5β,19-epoxycucurbita-6,23 E-diene-3β,25-diol( 8 ) and 5β,19-epoxycucurbita-6,23-diene-3β,19,25-triol( 9 ). The compounds were identified and elucidated by spectral and chemical methods. In addition, they were tested for their cytotoxicity against six cancer cell lines by MTT assay. Test solutions were given to cells in various final concentrations such as 0, 1, 10, 50, 100 μmol/L. The cytotoxic potential of the isolated compounds was investigated by determining the concentrations required for 50% growth inhibition (IC 50 value). Compounds 1 and 7 showed cytotoxicity. Compound 7 exhibited little cytotoxicity towards Du145 prostatic carcinoma cell line (IC 50 61.36 μmol/L), MCF-7 mammary adenocarcinoma cell line (IC 50 30.56 μmol/L), HL-60 leukemic cell line (IC 50 23.63 μmol/L), HGC gastric carcinoma cell line (IC 50 50.96 μmol/L), Colon205 colon carcinoma cell line (IC 50 34.49 μmol/L) and HepG2 hepatoma carcinoma cell line (IC 50 41.69 μmol/L). Compound 1 showed cytotoxicity only towards MCF-7 (IC 50 41.74 μmol/L). The remaining compounds showed no cytotoxicity.
Source: Planta Medica
Institutions: Yanbian University, Shenyang Pharmaceutical University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: bronze
Funders: National Science Foundation, U.S. Department of Agriculture, Wake Forest University, Western Carolina University, University Grants Commission, National Natural Science Foundation of China, Chinese Academy of Sciences, Hong Kong Polytechnic University, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, University of British Columbia, China Academy of Traditional Chinese Medicine, Tata Trusts, Deutsche Krebshilfe, Kurukshetra University, University of Colombo, International Science Council, National Institutes of Health, U.S. Food and Drug Administration, National Oceanic and Atmospheric Administration, University of Illinois at Urbana-Champaign, Agricultural Research Service, University of Illinois at Chicago, National Science Foundation of Sri Lanka, National Institute on Drug Abuse, National Institute of Allergy and Infectious Diseases, National Center for Complementary and Alternative Medicine, National Center for Research Resources
OpenAlex ID: W1981721760