Work

Protein sequencing by tandem mass spectrometry.

Year: 1986

Type: article

Abstract: Methodology for determining amino acid sequences of proteins by tandem mass spectrometry is described. The approach involves enzymatic and/or chemical degradation of the protein to a collection of peptides which are then fractionated by high-performance liquid chromatography. Each fraction, containing as many as 10-15 peptides, is then analyzed directly, without further purification, by a combination of liquid secondary-ion/collision-activated dissociation mass spectrometry on a multianalyzer instrument. Interpretation of collision-activated dissociation mass spectra is described, and results are presented from a study of soluble peptides produced by treatment of apolipoprotein B with cyanogen bromide and trypsin.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.83.17.6233

Authors: D F Hunt, J R Yates, J Shabanowitz, S Winston, C R Hauer

Institution: University of Virginia

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institutes of Health

Award: G1490403883