Insulin Receptors in the Liver: Specific Binding of [ 125 I]Insulin to the Plasma Membrane and Its Relation to Insulin Bioactivity
Year: 1971
Type: article
Abstract: With [(125)I]insulin at 7 x 10(-10) M, 25% of the radioactivity was bound to plasma membranes purified from rat liver. 20% of the [(125)I]insulin binding was inhibited by unlabeled insulin at 10(-9) M (6 ng/ml), equivalent to insulin concentrations in hepatic portal blood; inhibition of [(125)I]insulin binding was 80% at 10(-7) M and 90% at 10(-5) M. Eight insulins and derivatives with biological potencies that differed over a 100-fold range inhibited the binding of [(125)I]insulin to liver membranes in direct proportion to their ability to stimulate glucose oxidation in isolated fat cells. Inactive insulin chains, as well as glucagon, ACTH, and human growth harmone were without effect. The binding of [(125)I]insulin increased 55-fold as plasma membrane was purified from crude homogenate. Binding was time- and temperature-dependent, and addition of excess insulin produced rapid dissociation of [(125)I]insulin. This study demonstrates directly the binding of insulin to its biologically important receptors.
Authors: Pierre Freychet, Jesse Roth, David M. Neville
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W1982076963