Work

Crosstalk between bacterial chemotaxis signal transduction proteins and regulators of transcription of the Ntr regulon: evidence that nitrogen assimilation and chemotaxis are controlled by a common phosphotransfer mechanism.

Year: 1988

Type: article

Abstract: We demonstrate by using purified bacterial components that the protein kinases that regulate chemotaxis and transcription of nitrogen-regulated genes, CheA and NRII, respectively, have cross-specificities: CheA can phosphorylate the Ntr transcription factor NRI and thereby activate transcription from the nitrogen-regulated glnA promoter, and NRII can phosphorylate CheY. In addition, we find that a high intracellular concentration of a highly active mutant form of NRII can suppress the smooth-swimming phenotype of a cheA mutant. These results argue strongly that sensory transduction in the Ntr and Che systems involves a common protein phosphotransfer mechanism.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.85.15.5492

Authors: A J Ninfa, E G Ninfa, A N Lupas, A Stock, B Magasanik, J Stock

Institution: Princeton University

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Damon Runyon Cancer Research Foundation, National Institute of Allergy and Infectious Diseases

Award: MECHANISMS OF SENSORY PROCESSING IN CHEMOTAXIS