Work

Supercharged green fluorescent proteins as bimodal reporter genes for CEST MRI and optical imaging

Year: 2015

Type: article

Abstract: Superpositively charged mutants of green fluorescent protein (GFP) demonstrated a dramatically improved chemical exchange saturation transfer (CEST) MRI contrast compared to their wild type counterparts. The mutants +36 GFP and +48 GFP were successfully expressed in mammalian cells and retained part of their fluorescence, making them a new potential bimodal reporter gene.

Source: Chemical Communications

DOI: 10.1039/c4cc10195b

Authors: Amnon Bar-Shir, Yajie Liang, Kannie W. Y. Chan, Assaf A. Gilad, Jeff W. M. Bulte

Institutions: Johns Hopkins University, Johns Hopkins Medicine, Kennedy Krieger Institute

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Neurological Disorders and Stroke, National Institute of Biomedical Imaging and Bioengineering

Awards: Developing MRI sensors for monitoring Zn2+ using iCEST, G269535760, G283024544, MR and BL Tracking of Stem/Progenitor Cells in the CNS, G9000265697