Work

Improving sensitivity in nonlinear Raman microspectroscopy imaging and sensing

Year: 2011

Type: article

Abstract: Nonlinear Raman microspectroscopy based on a broadband coherent anti-Stokes Raman scattering is an emerging technique for noninvasive, chemically specific, microscopic analysis of tissues and large population of cells and particles. The sensitivity of this imaging is a critical aspect of a number of the proposed biomedical application. It is shown that the incident laser power is the major parameter controlling this sensitivity. By careful optimizing the laser system, the high-quality vibrational spectra acquisition at the multi-kHz rate becomes feasible.

Source: Journal of Biomedical Optics

DOI: 10.1117/1.3533317

Authors: Rajan Arora, Georgi I. Petrov, Vladislav V. Yakovlev, Jian Liu

Institutions: University of Wisconsin–Milwaukee, PolarOnyx (United States)

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Science Foundation, National Institutes of Health, National Eye Institute, National Institute of Biomedical Imaging and Bioengineering

Awards: Real-time, background-free resonance Raman microscopy FOR live-cell imaging, High-throughput vibrational cytometry, G657155060, Collaborative research: Disorder-induced high sensitivity Raman sensor