Work

Effects of Modified Digestion Schemes on the Identification of Proteins from Complex Mixtures

Year: 2006

Type: article

Abstract: In shotgun proteomics, a complex protein mixture is digested to peptides, separated, and identified by microcapillary liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). In this technology, complete protein digestion is often assumed. We show that, to the contrary, modifications to a standard digestion protocol demonstrate large, reproducible improvements in protein identification, a result consistent with digestion being a limiting factor in the efficiency of protein identification.

Source: Journal of Proteome Research

DOI: 10.1021/pr050315j

Authors: Aaron A. Klammer, Michael J. MacCoss

Institution: University of Washington

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Human Genome Research Institute, National Institute of General Medical Sciences, National Center for Research Resources

Awards: Training Program in Genetics General and Medical, Comprehensive Biology: Exploiting the Yeast Genome, Comprehensive Biology: Exploiting the Yeast Genome, Interdisciplinary Training in Genome Sciences