Work

Challenges for Standardization of Clostridium difficile Typing Methods

Year: 2013

Type: article

Abstract: Typing of Clostridium difficile facilitates understanding of the epidemiology of the infection. Some evaluations have shown that certain strain types (for example, ribotype 027) are more virulent than others and are associated with worse clinical outcomes. Although restriction endonuclease analysis (REA) and pulsed-field gel electrophoresis have been widely used in the past, PCR ribotyping is the current method of choice for typing of C. difficile. However, global standardization of ribotyping results is urgently needed. Whole-genome sequencing of C. difficile has the potential to provide even greater epidemiologic information than ribotyping.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.00143-13

Authors: Charlotte A. Huber, Niki F. Foster, Thomas V. Riley, David L. Paterson

Institutions: The University of Queensland, Royal Brisbane and Women's Hospital, The University of Western Australia

Language: English

Topic: Clostridium difficile and Clostridium perfringens research

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W1983174598

FWCI: 5.831

Cites: 30

Cited by: 63

Related to: 10