Work

A rapid detection method for PAI-1 promoter insertion/deletion polymorphism (4G/5G)

Year: 1998

Type: article

Abstract: Plasminogen activator inhibitor-1 (PAI-1) is an important inhibitor of fibrinolysis, and increased levels of PAI-1 are associated with atheroma and myocardial infarction. A common 4G/5G insertion/deletion polymorphism located in the promoter region of PAI-1 gene has been described associated with PAI-1 activity in plasma levels. Genotyping of this polymorphism is commonly conducted with an allele-specific oligonucleotide melting technique. In the present study, we describe a quick, easy method for genotyping 4G/5G polymorphism in the promoter region of the PAI-1 gene.

Source: Genetics and Molecular Biology

DOI: 10.1590/s1415-47571998000300004

Authors: Joyce M. Annichino-Bizzacchi, Luciana Pugliese, Valder R. Arruda

Institution: Universidade Estadual de Campinas (UNICAMP)

Language: English

Topic: Blood Coagulation and Thrombosis Mechanisms

Subfield: Hematology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $700