Work

Separation of Tricomponent Protein Mixtures with Triblock Nanorods

Year: 2006

Type: article

Abstract: Two-component triblock magnetic nanorods with gold end blocks and nickel interior blocks have been synthesized and used as affinity templates for the simultaneous and efficient separation of a three-component protein mixture. The gold blocks were selectively functionalized with 11-amino-1-undecanethiol, and then glutaraldehyde was used to covalently attach nitrostreptavidin to them. His-tagged proteins bind to the nickel block and biotin-tagged proteins bind to the functionalized gold ends, allowing one to separate a mixture of three proteins with a single material. Each surface-bound protein can be released selectively using imidazole for the His-tagged protein and biotin for the biotinylated protein.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja057525h

Authors: Byung-Keun Oh, Sungho Park, Jill E. Millstone, Seung Woo Lee, Ki-Bum Lee, Chad A. Mirkin

Institutions: Northwestern University, International Institute for Nanotechnology

Language: English

Topic: Protein purification and stability

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institutes of Health

Award: G9000165630