Work

Monitoring ATP hydrolysis and ATPase inhibitor screening using 1 H NMR

Year: 2014

Type: article

Abstract: We present a versatile method to characterize ATPase and kinase activities and discover new inhibitors of these proteins. The proton NMR-based assay directly monitors ATP turnover and is easy to implement, requires no additional reagents and can potentially be applied to GTP. We validated the method's accuracy, applied it to the monitoring of ATP turnover by actin and to the screening of ATPase inhibitors, and showed that it is also applicable for the monitoring of GTP hydrolysis.

Source: Chemical Communications

DOI: 10.1039/c4cc04399e

Authors: Bingqian Guo, Pinar S. Gurel, Rui Shu, Henry N. Higgs, Maria Pellegrini, Dale F. Mierke

Institution: Dartmouth College

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: Institut National de la Santé et de la Recherche Médicale, National Cancer Institute, National Institute of General Medical Sciences