Work

IgM and stromal cell-associated heparan sulfate / heparin as complement-independent ligands for CD19

Year: 2001

Type: article

Abstract: The more severe phenotype of mice lacking CD19 as compared to CD21 suggests that a complement-independent ligand for the CD19/CD21 complex exists. We sought ligands for CD19 by examining binding reactions with fusion proteins comprised of the extracellular region of CD19 and the Fc region of IgG1. A fusion protein containing the third extracellular domain (D3-Fc) bound to WEHI-231 cells, and this was competed by soluble IgM. This function of IgM was confirmed by the binding of D3-Fc to beads coated with IgM. A second ligand for D3-Fc was found on stromal cells, and was shown to be heparin/heparan sulfate. These two ligands would be considered to reside on follicular dendritic cells, and may account for the observed ability of D3-Fc to bind to sites in germinal centers containing these cells.

Source: European Journal of Immunology

DOI: 10.1002/1521-4141(200107)31:7<2189::aid-immu2189>3.0.co;2-v

Authors: Antonin R. de Fougerolles, Facundo Batista, Eskil Johnsson, Douglas T. Fearon

Institutions: University of Cambridge, Wellcome Trust, MRC Laboratory of Molecular Biology, Medical Research Council

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Wellcome Trust