Work

Label and Label-Free Detection Techniques for Protein Microarrays

Year: 2015

Type: article

Abstract: Protein microarray technology has gone through numerous innovative developments in recent decades. In this review, we focus on the development of protein detection methods embedded in the technology. Early microarrays utilized useful chromophores and versatile biochemical techniques dominated by high-throughput illumination. Recently, the realization of label-free techniques has been greatly advanced by the combination of knowledge in material sciences, computational design and nanofabrication. These rapidly advancing techniques aim to provide data without the intervention of label molecules. Here, we present a brief overview of this remarkable innovation from the perspectives of label and label-free techniques in transducing nano‑biological events.

Source: Microarrays

DOI: 10.3390/microarrays4020228

Authors: Amir Syahir, Kenji Usui, Kin-ya Tomizaki, Kotaro Kajikawa, Hisakazu Mihara

Institutions: Universiti Putra Malaysia, Konan University, Ryukoku University, Tokyo Institute of Technology

Language: English

Topic: Advanced Biosensing Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $378

Funders: Ministry of Education, Culture, Sports, Science and Technology, Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering, Japan Society for the Promotion of Science