Work

Amphetamine releases dopamine from synaptic vesicles by dual mechanisms

Year: 1996

Type: article

Abstract: Low concentrations of D-amphetamine (AMPH) released [3H]dopamine (DA) from purified, rat brain synaptic vesicles with exponential kinetics and a half-time of 4 min. Slight alkalinization of the vesicles by AMPH seemed unable to account for this release. Instead, it is likely that AMPH partially inhibits DA uptake after which DA escapes by an uptake-independent pathway. At high concentrations the proton gradient was severely reduced by AMPH and DA was released with a half-time of < 1 min. Loss of the proton gradient was sufficient to account for this DA release. These results show that low concentrations of AMPH release DA from synaptic vesicles and suggest that release occurs by different mechanisms at low and high AMPH concentrations.

Source: Neuroscience Letters

DOI: 10.1016/s0304-3940(96)12963-3

Authors: Erik Floor, Lihao Meng

Institution: University of Kansas

Language: English

Topic: Neuroscience and Neuropharmacology Research

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

Funders: National Institute on Aging, National Institute of Neurological Disorders and Stroke