Construction of Differential-Methylation Subtractive Library

Year: 2014

Type: article

Abstract: Stress-induced ROS changes DNA methylation patterns. A protocol combining methylation-sensitive restriction endonuclease (MS-RE) digestion with suppression subtractive hybridization (SSH) to construct the differential-methylation subtractive library was developed for finding genes regulated by methylation mechanism under cold stress. The total efficiency of target fragment detection was 74.64%. DNA methylation analysis demonstrated the methylation status of target fragments changed after low temperature or DNA methyltransferase inhibitor treatment. Transcription level analysis indicated that demethylation of DNA promotes gene expression level. The results proved that our protocol was reliable and efficient to obtain gene fragments in differential-methylation status.

Source: Journal of Chemistry

DOI: 10.1155/2014/536134

Authors: Wei Hu, Xiaolei Liang, Tian Dong, Yanfeng Hu, Jie Liu, Lina Mao, Xu Liu, Yurong Bi

Institutions: Lanzhou University, Chinese Academy of Sciences, Northeast Institute of Geography and Agroecology

Language: English

Topic: Epigenetics and DNA Methylation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,160

Funders: National Natural Science Foundation of China, Major State Basic Research Development Program of China, National Key Research and Development Program of China, Fundamental Research Funds for the Central Universities, Science and Technology Program of Gansu Province

OpenAlex ID: W1984096399