Work

Pre‐mRNA 3′‐end processing complex assembly and function

Year: 2010

Type: review

Abstract: The 3'-ends of almost all eukaryotic mRNAs are formed in a two-step process, an endonucleolytic cleavage followed by polyadenylation (the addition of a poly-adenosine or poly(A) tail). These reactions take place in the pre-mRNA 3' processing complex, a macromolecular machinery that consists of more than 20 proteins. A general framework for how the pre-mRNA 3' processing complex assembles and functions has emerged from extensive studies over the past several decades using biochemical, genetic, computational, and structural approaches. In this article, we review what we have learned about this important cellular machine and discuss the remaining questions and future challenges.

Source: Wiley Interdisciplinary Reviews - RNA

DOI: 10.1002/wrna.54

Authors: Serena Chan, Eun‐A Choi, Yongsheng Shi

Institution: University of California, Irvine

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: Characterization of the mammalian mRNA 3'-end processing complex