Work

Enumeration of Viable Listeria monocytogenes Cells by Real-Time PCR with Propidium Monoazide and Ethidium Monoazide in the Presence of Dead Cells

Year: 2007

Type: article

Abstract: Propidium monoazide (PMA) and ethidium monoazide were used for enumeration of viable Listeria monocytogenes cells in the presence of dead cells. PMA had no antimicrobial effect on L. monocytogenes. Viable cell counts were linearly related to real-time PCR threshold cycle values for PMA-treated cells from planktonic and biofilm sources over a 4-log range.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.01198-07

Authors: Y. Pan, F. Breidt

Institutions: Agricultural Research Service, North Carolina State University

Language: English

Topic: Listeria monocytogenes in Food Safety

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W1984770343

FWCI: 14.05

Cites: 16

Cited by: 280

Related to: 10