Year: 2014
Type: article
Abstract: Compared with visible light (380-700 nm), near-infrared light (700-1400 nm) undergoes weaker optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared optical-resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using optical fluence within the American National Standards Institute (ANSI) limit (100 mJ/cm2). Beyond ∼0.6 mm deep in chicken breast tissue, NIR-OR-PAM has shown finer resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.
Source: Optics Letters
DOI: 10.1364/ol.39.005192
Institution: Washington University in St. Louis
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
Awards: Photon tunneling: Shedding new light on biomedicine, G1952371934, Collaborative Research/IDBR: High-Throughput Measurement of Oxygen Consumption Rates of Single Cells Using Wide-Field Optical-Resolution Photoacoustic Microscopy, In vivo imaging of single circulating cells, PHOTOACOUSTIC MICROSCOPY OF NEOVASCULARIZATION IN RENAL CANCER GROWTH AND THERAPY
OpenAlex ID: W1984875190