Work

Immune reactions in skin and hair follicle gene therapy

Year: 2003

Type: article

Abstract: An immunological response to non-native proteins is aproblem that must be overcome for successful gene therapy. A study by Ghazizadeh et al. in this issue of Molecular Therapy describes the immune response to ret-rovirus-mediated lacZ gene transfer to the skin [1]. The study shows that both CD4 and CD8 immune responses need to be eliminated to permit persistent expression of this transgene. The investigators use of transgenic mice lacking both CD4 and CD8 function was critical for this study. Following retroviral delivery of lacZ to the dorsal skin of immunocompetant B6 mice and mice immunodefi-cient for the immunoglobulin heavy chain (Igh/), fro-zen tissues sections were prepared and analyzed by X-gal staining to detect transduced cells. Two weeks after trans-duction with the virus, no X-gal positive cells were ob-

Source: Molecular Therapy

DOI: 10.1016/s1525-0016(03)00040-6

Author: Robert M Hoffman

Institution: AntiCancer (United States)

Language: English

Topic: Virus-based gene therapy research

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,500