An Important Role for Mitochondrial Antiviral Signaling Protein in the Kaposi's Sarcoma-Associated Herpesvirus Life Cycle
Year: 2014
Type: article
Abstract: UNLABELLED: Kaposi's sarcoma-associated herpesvirus (KSHV) has been shown to be recognized by two families of pattern recognition receptors (PRRs), Toll-like receptors (TLRs) and NOD-like receptors (NLRs). Here we show that MAVS and RIG-I (retinoic acid-inducible gene 1), an RLR family member, also have a role in suppressing KSHV replication and production. In the context of primary infection, we show that in cells with depleted levels of MAVS or RIG-I, KSHV transcription is increased, while beta interferon (IFN-β) induction is attenuated. We also observed that MAVS and RIG-I are critical during the process of reactivation. Depletion of MAVS and RIG-I prior to reactivation led to increased viral load and production of infectious virus. Finally, MAVS depletion in latent KSHV-infected B cells leads to increased viral gene transcription. Overall, this study suggests a role for MAVS and RIG-I signaling during different stages of the KSHV life cycle. IMPORTANCE: We show that RIG-I and its adaptor protein, MAVS, can sense KSHV infection and that these proteins can suppress KSHV replication following primary infection and/or viral reactivation.
Source: Journal of Virology
DOI: 10.1128/jvi.03226-13
Authors: John A. West, Megan Wicks, Sean M. Gregory, Pauline Chugh, Sarah R. Jacobs, Zhigang Zhang, Kurtis M. Host, Dirk P. Dittmer, Blossom Damania
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
Awards: G1111229574, G1178797104, G1393734537, G1465922187, G1646449232, G1657955723, G1694141453, G1741978266, G1783224161, G1815638046, G222337112, Discovery of New Innate Immune Pathways in Viral Recognition, G38106945, G3860392808, Innate Immunity and KSHV, KSHV Modulation of the Host Innate Immune System, G5331442387, G9000023225, G9000191358, G9000210637, G9000215696, The University of North Carolina Center for AIDS Research, ART Modulation of Viral Pathogenesis
OpenAlex ID: W1985240283