Work

Chromatin assembly on replicating DNAin vitro

Year: 1990

Type: article

Abstract: Replicating single-stranded DNA is preferentially assembled into chromatin in Xenopus egg extracts relative to non-replicating double-stranded DNA. We have examined the molecular basis of this phenomenon. Single-stranded DNA itself is not a favored template for nucleosome assembly in comparison to double-stranded DNA. Complementary strand synthesis is required for the rapid assembly of nucleosomes. We present evidence that the assembly of chromatin on replicating DNA is a two step phenomenon. The first step involves the replication of DNA and the assembly of an intermediate structure, the second step involves the sequestration of histones H2A/H2B onto DNA. Histones H2A/H2B are preferentially sequestered onto replicated DNA in comparison to non-replicated DNA incubated in the extract.

Source: Nucleic Acids Research

DOI: 10.1093/nar/18.19.5767

Authors: Geneviéve Almouzni, David J. Clark, Marcel Méchali, Alan P. Wolffe

Institutions: Centre National de la Recherche Scientifique, National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases, Institut Jacques Monod

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W1985352832

FWCI: 3.138

Cites: 32

Cited by: 110

Related to: 10