Work

Functional Delivery of a Membrane Protein into Oocyte Membranes Using Bicelles

Year: 2010

Type: article

Abstract: Voltage-gated potassium channel modulatory membrane protein KCNE3 was overexpressed and purified into both micelles and bicelles. Remarkably, microinjection of KCNE3 in bicelles into Xenopus oocytes resulted in functional co-assembly with the human KCNQ1 channel expressed therein. Microinjection of LMPC micelles containing KCNE3 did not result in channel modulation, indicating that bicelles sometimes succeed at delivering a membrane protein into a cellular membrane when classical micelles fail. Backbone NMR resonance assignments were completed for KCNE3 in both bicelles and LMPC, indicating that the secondary structure distribution in KCNE3's N-terminus and transmembrane domains exhibits only modest differences from that of KCNE1, even though these KCNE family members have very different effects on KCNQ1 channel function.

Source: Biochemistry

DOI: 10.1021/bi902155t

Authors: Congbao Kang, Carlos G. Vanoye, Richard C. Welch, Wade D. Van Horn, Charles R. Sanders

Institution: Vanderbilt University

Language: English

Topic: Lipid Membrane Structure and Behavior

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institute on Deafness and Other Communication Disorders, National Institute of Neurological Disorders and Stroke

Awards: G123344359, G7183911145