Work

Tissue- and site-specific DNA recombination in transgenic mice.

Year: 1992

Type: article

Abstract: We have developed a method of specifically modifying the mammalian genome in vivo. This procedure comprises heritable tissue-specific and site-specific DNA recombination as a function of recombinase expression in transgenic mice. Transgenes encoding the bacteriophage P1 Cre recombinase and the loxP-flanked beta-galactosidase gene were used to generate transgenic mice. Genomic DNA from doubly transgenic mice exhibited tissue-specific DNA recombination as a result of Cre expression. Further characterization revealed that this process was highly efficient at distinct chromosomal integration sites. These studies also imply that Cre-mediated recombination provides a heritable marker for mitoses following the loss of Cre expression. This transgene-recombination system permits unique approaches to in vivo studies of gene function within experimentally defined spatial and temporal boundaries.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.89.15.6861

Authors: P C Orban, D Chui, J D Marth

Institution: University of British Columbia

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Medical Research Council Canada, Medical Research Council, National Cancer Institute

OpenAlex ID: W1985634042

FWCI: 4.917

Cites: 24

Cited by: 672

Related to: 10