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Analysis of murine interferon‐γ binding to its receptor on intact cells and solubilized membranes Identification of an 80 kDa receptor

Year: 1989

Type: article

Abstract: The receptor for murine-interferon-gamma (Mu-IFN-gamma) has been characterized for its molecular size and equilibrium binding constant on a thymoma cell line, EL-4. Binding of 125I-IFN-gamma to intact cells and their solubilized membranes has shown a single class of receptor with Kd values of 1.9 x 10(-9) M and 1.3 x 10(-8) M, respectively. It was shown that solubilization of the Mu-IFN-gamma receptor with a Zwitterionic detergent (Chaps) preserves its binding activity. A direct comparison of the molecular mass of the Mu-IFN-gamma receptor on intact cells versus detergent-solubilized membranes was performed using a radiolabeled photoactivated crosslinking reagent and direct hybridization with 125I-labeled IFN-gamma on Western blots of solubilized receptor. The results indicate that both types of receptors have an identical molecular mass of approximately 80 kDa.

Source: FEBS Letters

DOI: 10.1016/0014-5793(89)80475-2

Authors: F. Cofano, S. Landolfo, ]E. Appella, S.J. Ullrich

Institutions: National Cancer Institute, National Institutes of Health, University of Turin

Language: English

Topic: Cytokine Signaling Pathways and Interactions

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

OpenAlex ID: W1986554545

FWCI: 0.517

Cites: 14

Cited by: 6

Related to: 10