Year: 1993
Type: preprint
Abstract: Prostate cancer is now the most commonly diagnosed cancer in males, with mortality approaching 25%.r Allelic loss, or loss of heterozygosity (LOH), of chromosomal regions 8p, 10q, and 16q are observed frequently in clinically evident prostate tumors, r suggesting that they harbor tumor suppressor genes critical to the suppression of prostate tumor induction and progression.To elucidate the role of LOH for these and other chromosomal regions in prostate tumorigenesis, the frequency of these events must be examined in clinically latent, or "histologic" cancers, as well as in normal and premalignant glandular epithelial tissue from prostates without evidence of cancer.These tissues are difficult to obtain from surgical material but may be obtained from autopsy material.However, DNA isolated from autopsy-obtained tissue is difficult to analyze genetically because it is invariably degraded due to (1) fixation in formalin, which induces acid hydrolysis of DNA and protein-DNA cross-linking; and (2) autolysis, which may vary in severity depending on the interval between time of death and tissue procurement.In this paper we report a PCR-based technique that overcomes these obstacles by targeting nucleotide repeat polymorphic sequences to the chromosomal loci of interest in degraded DNA purified from formalin-fixed, paraffin-embedded autopsy tissue.
Source: Genome Research
DOI: 10.1101/gr.2.4.354
Authors: J A Macoska, P D Benson, L N Turkeri, G P Haas, W Sakr
Institution: Wayne State University
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W1986696128