Work

Actin-driven cell dynamics probed by Fourier transform light scattering

Year: 2010

Type: article

Abstract: We applied the newly developed Fourier transform light scattering (FTLS) to study dynamic light scattering in single live cells, at a temporal scale of seconds to hours. The nanoscale cell fluctuations were measured with and without the active actin contribution. We found experimentally that the spatio-temporal signals rendered by FTLS reveal interesting cytoskeleton dynamics in glial cells (the predominant cell type in the nervous system). The active contribution of actin cytoskeleton was obtained by modulating its dynamic properties via Cytochalasin-D, a drug that inhibits actin polymerization/depolymerization.

Source: Biomedical Optics Express

DOI: 10.1364/boe.1.000260

Authors: Huafeng Ding, Larry J. Millet, Martha U. Gillette, Gabriel Popescu

Institution: University of Illinois Urbana-Champaign

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, Grainger Foundation, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Awards: G3574915600, G5478703346, G6671297155