Work

Classification of DNA sequences based on thermal melting profiles

Year: 2010

Type: article

Abstract: A new classification scheme based on the melting profile of DNA sequences simulated thermal melting profiles. This method was applied in the classification of (a) several species of mammalian - β globin and (b) α-chain class II MHC genes. Comparison of the thermal melting profile with the molecular phylogenetic trees constructed using the sequences shows that the melting temperature based approach is able to reproduce most of the major features of the sequence based evolutionary tree. Melting profile method takes into account the inherent structure and dynamics of the DNA molecule, does not require sequence alignment prior to tree construction, and provides a means to verify the results experimentally. Therefore our results show that melting profile based classification of DNA sequences could be a useful tool for sequence analysis.

Source: Bioinformation

DOI: 10.6026/97320630004463

Authors: Edward Reese, V. V. Krishnan

Institutions: California State University, Fresno, University of California Davis Medical Center, University of California, Davis

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funders: National Institutes of Health, National Institute on Minority Health and Health Disparities

Award: Developing Biomedical Research Infrastructure for California's Central Valley