Work

The Structure of Plant Cell Walls: III. A Model of the Walls of Suspension-cultured Sycamore Cells Based on the Interconnections of the Macromolecular Components

Year: 1973

Type: article

Abstract: Degradative enzymes have been used to obtain defined fragments of the isolated cell walls of suspension-cultured sycamore cells. These fragments have been purified and structurally characterized. Fragments released from endopolygalacturonase-pretreated cell walls by a purified endoglucanase and the fragments extracted from these walls by urea and alkali provide evidence for a covalent connection between the xyloglucan and pectic polysaccharides. Fragments released by a protease from endopolygalacturonase-endoglucanase-pretreated cell walls provide evidence for a covalent connection between the pectic polysaccharides and the structural protein of the cell wall. Based on these interconnections and the strong binding which occurs between the xyloglucan and cellulose, a tentative structure of the cell wall is proposed.

Source: PLANT PHYSIOLOGY

DOI: 10.1104/pp.51.1.188

Authors: Kenneth Keegstra, Kenneth W. Talmadge, W. D. Bauer, Peter Albersheim

Institutions: University of Colorado Boulder, Princeton University, Michigan State University

Language: English

Topic: Polysaccharides and Plant Cell Walls

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: bronze

Funders: National Science Foundation, Massachusetts Institute of Technology

OpenAlex ID: W1987188659