Placental Hofbauer cells assemble and sequester HIV-1 in tetraspanin and DC SIGN positive compartments that are accessible to neutralizing antibodies
Year: 2014
Type: article
Abstract: Within monocyte derived macrophages, HIV-1 accumulates in virus containing compartments (VCCs) that are largely inaccessible to the external environment, which implicate these cells as HIV-1 reservoirs. During mother to child transmission, placental macrophages (Hofbauer Cells [HCs]) are viral targets, and have shown to be infected in vivo and sustain low levels of viral replication in vitro , however, the risk of in utero transmission is less than 7%. The role of these primary macrophages as viral reservoirs is largely undefined. With consent, term placentas from 20 HIV-1 seronegative women were obtained following caesarian section. VCCs were evaluated with 3D confocal microscopy and correlated with electron microscopy. Co localization R values (Pearson’s correlation) were quantified with co localization module of Volocity5.2.1. Replication kinetics following siRNA and neutralization studies were evaluated using p24 ELISA. We demonstrate primary HCs assemble and sequester HIV-1 in VCCs with localization specific to intracellular vesicles and the plasma membrane. These compartments are enriched in endosomal/lysosomal markers, including CD9, CD81, CD63 and LAMP 1, along with DC SIGN. Following internalization, we observed HIV-1 accumulation in acidified compartments. Remarkably, these compartments are accessible via the cell surface and can be targeted by exogenously applied small molecules and HIV-1 specific neutralizing antibodies (NAbs). In addition, broadly NAbs (4E10 and VRC01) neutralized HIV-1 infected HCs and involve the participation of FcγRI for inhibition. These findings suggest placental HCs possess intrinsic adaptations facilitating sequestration of HIV-1, and may serve as a protective viral reservoir to permit viral degradation, neutralization and antiretroviral drug entry in utero .
Source: BMC Infectious Diseases
Authors: Erica L Johnson, Hin Chu, Siddappa N Byrareddy, Paul Spearman, Rana Chakraborty
Institutions: Emory University, Children's Healthcare of Atlanta
Language: English
Topic: HIV Research and Treatment
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,090
OpenAlex ID: W1988378969