Work

H/D Exchange Centroid Monitoring is Insufficient to Show Differences in the Behavior of Protein States

Year: 2013

Type: article

Abstract: Differential hydrogen/deuterium exchange (H/DX) coupled with mass spectrometry (H/DX-MS) offers a rapid and sensitive characterization of changes in proteins following perturbations induced by changes in folding, ligand binding, oligomerization, and modification. The characterization of H/DX rates by software tools and automated data processing often relies on the centroid mass calculation and, thereby, the deuterium distribution in the mass spectra is neglected. Here we present an example demonstrating the clear limitation of using only a centroid approach to characterize the H/DX rate, in which the change in protein is not reflected as the difference in deuterium uptake based on centroid calculation.

Source: Journal of the American Society for Mass Spectrometry

DOI: 10.1007/s13361-012-0555-z

Authors: Jun Zhang, Pradeep Ramachandran, Rajiv Kumar, Michael L. Gross

Institutions: Washington University in St. Louis, Mayo Clinic

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: A Biomedical Mass Spectrometry Resource: Ongoing Driving Biomedical Projects, A Resource for Biomedical Mass Spectrometry