Work

RARγ is required for correct deposition and removal of Suz12 and H2A.Z in embryonic stem cells

Year: 2010

Type: article

Abstract: Retinoic acid (RA) induces embryonic stem cell differentiation. The effects of RA are mediated by retinoic acid receptors (RARs) that promote epigenetic changes controlling gene transcription. We show here that RARγ, in the absence of the ligand RA, is required for deposition of the histone variant H2A.Z and the polycomb group protein Suz12 at RA target genes, and that in embryonic stem cells both RARγ and Suz12 exist in a multi-protein complex in the absence of ligand. Addition of RA causes removal of H2A.Z and Suz12 from RARγ target genes when the genes are transcriptionally activated.

Source: Journal of Cellular Physiology

DOI: 10.1002/jcp.22420

Authors: Ramon Amat, Lorraine J. Gudas

Institutions: Cornell University, Weill Cornell Medicine

Language: English

Topic: Epigenetics and DNA Methylation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute

Award: G12547682