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Heterologous in vitro transcription from two archaebacterial promoters

Year: 1991

Type: article

Abstract: A cell-free extract of Sulfolobus shibatae is able to specifically initiate transcription in vitro at the promoter of the plasmid-encoded gene for the major gas vesicle protein of Halobacterium halobium and at the promoter for the transcript T4 of the temperate H. halobium phage phi H. The corresponding promoter from the virulent phage mutant phi HL1 yields enhanced transcription in the heterologous system, in agreement with strongly increased in vivo expression.

Source: FEBS Letters

DOI: 10.1016/0014-5793(91)80811-g

Authors: Uwe Hüdepohl, Felix Gropp, Mary Horne, Wolfram Zillig

Institutions: Max Planck Society, Max Planck Institute of Biochemistry, University of California, San Francisco

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W1989007973

FWCI: 1.069

Cites: 16

Cited by: 9

Related to: 10