Work

Construction and screening of vast libraries of natural product-like macrocyclic peptides using in vitro display technologies

Year: 2014

Type: review

Abstract: Macrocyclic structure and backbone N-methylation represent characteristic features of peptidic natural products, which play critical roles in their biological activity. Although natural products have been the traditional source of such peptides, recent developments in synthesizing natural product-like macrocyclic peptides using reconstituted translation systems have enabled us to construct vast trillion-member libraries of non-standard macrocyclic peptides. In addition, a method for displaying such libraries on their corresponding mRNA templates allows us to rapidly screen them for potent ligands against various drug targets. This review describes methodologies for the ribosomal synthesis of novel natural product-like macrocyclic peptides and their recent applications in the discovery of bioactive molecules using in vitro display technologies.

Source: Current Opinion in Chemical Biology

DOI: 10.1016/j.cbpa.2014.11.011

Authors: Nasir K Bashiruddin, Hiroaki Suga

Institution: The University of Tokyo

Language: English

Topic: Chemical Synthesis and Analysis

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,510

Funder: Ministry of Education, Culture, Sports, Science and Technology