Work

Assignment of the13C nuclear magnetic resonance spectrum of a short DNA-duplex with1H-detected two-dimensional heteronuclear correlation spectroscopy

Year: 1987

Type: article

Abstract: Proton-detected 1H-13C heteronuclear correlated spectroscopy [( 1H,13C]-COSY) was used to establish relations between the carbon-13 and proton nuclear magnetic resonance chemical shifts in the hexadeoxynucleoside pentaphosphate d-(GCATGC)2. Using the previously established sequence-specific proton NMR assignments, sequence-specific assignments were thus obtained for nearly all proton-bearing carbons. This approach offers a new criterion for distinguishing between the proton NMR lines of purines and pyrimidines, based on the different proton-carbon-13 coupling constants. Furthermore, the adenine ring carbon 2 has a unique carbon-13 chemical shift, which enables a straightforward identification of the adenine C2H resonances by [1H,13C]-COSY.

Source: Nucleic Acids Research

DOI: 10.1093/nar/15.1.267

Authors: Werner Leupin, Gerhard Wagner, William A. Denny, Kurt Wüthrich

Institutions: ETH Zurich, Institute of Molecular Biology and Biophysics, University of Auckland

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

OpenAlex ID: W1990367089

FWCI: 2.429

Cites: 9

Cited by: 29

Related to: 10