Year: 2012
Type: article
Abstract: Synthetic biology applications call for efficient methods to generate large gene cassettes that encode complex gene circuits in order to avoid simultaneous delivery of multiple plasmids encoding individual genes. Multiple methods have been proposed to achieve this goal. Here, we describe a novel protocol that allows one-step cloning of up to four gene-size DNA fragments, followed by a second assembly of these concatenated sequences into large circular DNA. The protocols described here comprise a simple, cheap and fast solution for routine construction of cassettes with up to 10 gene-size components.
Source: Nucleic Acids Research
DOI: 10.1093/nar/gks236
Authors: Jonathan Leo Schmid-Burgk, Zhen Xie, Stefan Frank, Sebastian Virreira Winter, Sibylle Mitschka, Waldemar Kolanus, Andrew Murray, Yaakov Benenson
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,992
Funder: National Cancer Institute
OpenAlex ID: W1991094235